Imaging - Institute of Biological Chemistry, Academia Sinica

The facilities provide advanced imaging systems including: fluorescence microscope; laser-scanning confocal microscopes; high-content screening station; ... Skiptocontent Imaging UserCommitteeMembers TakashiAngata、Hung-Yuan(Peter)Chi、Chung-IChang(Chair)、Guang-ChaoChen、Chin-ChunHung、Meng-ChiaoHo、Chun-HungLin、Hsiao-ChingLin、StevenLin Location Rm406 Contact Chin-ChunHung [email protected] (02)27855696#4022 Introduction TheBio-Imaging&CellBiologyFacilitiesislocatedatRoom406,InstituteofBiologicalChemistry,AcademiaSinica.ThefacilitiesaretoprovideIBCinvestigatorswiththedesignofimagingexperiments,choiceoftrainingonsuitableimagingsystems,andsubsequentimageprocessing,visualizationandanalysis. Thefacilitiesprovideadvancedimagingsystemsincluding:fluorescencemicroscope;laser-scanningconfocalmicroscopes;high-contentscreeningstation;flowcytometers;bioluminescenceandchemiluminescenceimager. 1.LeicaSP5XInvertedConfocalMicroscope Reservation(IBC) TheLeicaSP5XConfocalMicroscopeisequippedwithseverallaserlines,includinga405diodeandwhitelightlaser(470-670nm),amotorizedx-yscanningstage,z-galvostage.Suitableapplicationsincludelivecellimagingstudies,FRAP,FRET,timecourses,colocalizationandspectralunmixing. 2.LeicaDMI6000BInvertedMicroscope On-sitereservation TheLeicaDMI6000Binvertedmicroscopeisanautomatedlivecellimagingsystem.Thesystemissuitableforfluorescence,livecell,time-lapseimaging,high-speedmulti-fluorescenceopticalsectioning,andmicromanipulation. 3.Olympusscan^Rhigh-contentscreenstation On-sitereservation TheScan^Rscreeningplatformcanbeusedforassaydevelopmentandhigh-contentscreeningusingmultiwellplatesaswellassingleslides.Thismakesitpossibletoscreenbothlargeandsmallnumbersofsamples. 4.FujifilmLuminescentImageAnalyzerLAS4000 On-sitereservation TheLAS4000isamultipurposeCCDcamerasystemforsensitiveandquantitativeimagingofmembranes,gels,smallanimals,colonycounting,etc.Itisequippedwithstandardtrans-illuminationwhitelightandUVaswellasepi-illuminationwhitelight,red,greenandbluefluorescence. 5.OlympusFV3000RSInvertedConfocalMicroscope Reservation(IBC) TheOlympusFV3000RSInvertedConfocalMicroscopeisequippedwithseveraldiodelasers,including405,445,488,514,561,594and640nmlasers,amotorizedx-yscanningstage,withtwoultrasensitiveGaAsPdetectorsthatallowvisualizationofweakfluorescentsignalswithlowerphotobleachingandphototoxicity.Suitableapplicationsincludelivecellimagingstudies,FRAP,FRET,timecourses,colocalizationandspectralunmixing. 6.BeckmanCytoFLEX On-sitereservation BeckmanCytoFLEXFlowCytometerisequippedwithtwolasersprovideexcitationat488and638nmforanalysisinsixfluorescencechannelsplusforwardandsidescatter.Thisanalyzeriscapableofprovidingimmunophenotyping,absolutecounting,residualwhitebloodcellenumeration,stemcellanalysis,DNAanalysis. English 中文English AboutIBC IBCHistory Organization Directors AdvisoryBoardMembers Location PersonalInformationProtectionActDisclosure People Investigators PrincipalInvestigators Angata,Takashi Chang,Ching-Jin Chang,Chung-I Chen,Guang-Chao Chen,Hungwen Chen,Ruey-Hwa Chen,RitaP.-Y. Ho,Meng-Chiao Hsu,Shang-TeDanny Khoo,Kay-Hooi Liang,Po-Huang Liao,JamesC. Lin,Chun-Hung Lin,Hsiao-Ching Lin,Steven Lowary,ToddL. Meng,Tzu-Ching Shih,Yu-Ling Wang,Yane-Shih Weng,Jui-Hsia Wu,Kuen-Phon Wu,Shih-Hsiung Yang,WeiYuan Yao,Chi-Kuang JointAppointmentFaculty Chang,Mau-Sun Chi,Hung-Yuan(Peter) Hsiao,Chiaolong Kuan,Yung-Shu Wolf,Matthias Wong,Chi-Huey Yen,Yun Yu,Ching-Ching Yu,Lung-Chih CorrespondingResearchFellow EmeritusFaculty ResearchSpecialists Yuan-Chih(Kondo)Chang Ho,Meng-Ru Huang,Kai-Fa Hung,Chin-Chun Jao,Shu-Chuan(Chris) Lin,Sheng-Wei Lin,Shu-Yu Tu,Zhijay Wu,Wen-Jin VisitingScholars Bessho,Yoshitaka Maestre-Reyna,Manuel Tsai,Ming-Daw Wang,AndrewH.-J. Assistants AdministrativeStaff Research ResearchFields ResearchHighlights TraineeHighlight LatestPublications Facilities Protein Proteinpurificationandcentrifuge Mammaliancell-basedrecombinantproteinexpression Biophysics Proteincrystallization CellBiology Imaging Histopathology Animalroom Bioinformatics Chemistry Synthesis NuclearMagneticResonance High-PerformanceLiquidChromatography FacilityatNTUcampus ASfacility ProteomicsMassSpectrometryCommonFacility BiophysicsCoreFacility AcademiaSinicaCryo-EMFacility AcademiaSinicaProteinClinic Events IBCCalendar Seminars&Symposia News&Awards EventRecaps Education IBS,NTU TIGP-CBMB SummerInternshipProgram Jobs Intranet Links AcademiaSinica TaiwanProteinProject(TPP) AcademiaSinicaLifeScienceLibrary ActivityCenterBooking